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目的 评价健脾消渴方对2型糖尿病大鼠的药效，并探讨其可能的作用机制。方法 40只SD大鼠随机分为正常对照组、模型对照组、健脾消渴方组及盐酸二甲双胍组，每组10只，STZ+高脂饮食建立2型糖尿病大鼠模型，给予相应药物，持续4周。实验结束后取材，考察大鼠空腹血糖变化情况；ELISA法检测大鼠血清中白细胞介素-1(interleukin-1β,IL-1β)、白细胞介素-10(interleukin-10,IL-10)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、D-氨基酸氧化酶(D-amino acid oxidase,DAO)、D-乳酸含量；考察血清血脂四项[甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)]含量；HE染色法观察各组大鼠胰腺及回肠的组织形态学改变；免疫荧光法检测大鼠回肠L细胞的免疫荧光法检测大鼠回肠L细胞的胰高糖素样肽-1(glucagon-like peptide-1,GLP-1)阳性表达；Western blotting法检测大鼠结肠组织中紧密连接蛋白(occludin)、闭锁小带蛋白-1(zonula occludens-1,ZO-1)、黏蛋白2(Muc2)蛋白相对表达。结果 健脾消渴方可有效降低2型糖尿病大鼠的空腹血糖，下调血清中IL-1β、TNF-α含量，上调IL-10含量，抑制DAO、D-乳酸含量；同时，显著性下调TG、TC、LDL-C含量，但在提高HDL-C含量方面上仅具有趋势性；此外，健脾消渴方可改善大鼠胰腺及回肠损伤，提高GLP-1阳性表达，并有效降低2型糖尿病大鼠结肠occludin、ZO-1、Muc2蛋白相对表达。结论 健脾消渴方对2型糖尿病大鼠具有较好的治疗效果，其作用可能与改善血清炎症、调节血脂，减轻肠道功能损伤，改善肠道屏障通透性与保护肠道屏障生理结构等作用有关。
Abstract：Objective To evaluate the effect of Jianpi Xiaoke Decoction(健脾消渴方)on type 2diabetic rats and explore its possible mechanism. Methods 40 cases SD rats were randomly divided into normal control group,model control group,Jianpi Xiaoke Decoction group and positive medicine group,10 rats in each group. STZ+ high fat diet was used to establish type 2 diabetic rat model,and corresponding medicine was given for 4 weeks. After the end of the experiment,the rats were sampled to investigate the changes of fasting blood glucose;the expression levels of IL-1β,IL-10,TNF-α,DAO and D-lactic acid in serum and the changes of serum lipids(TG,TC,HDL-C and LDL-C)were detected by ELISA;the histomorphological changes of pancreas and ileum were observed by HE staining;the GLP-1 positive expression in L cells of ileum was detected by immunofluorescence method,and the relative expression of occludin,ZO-1 and Muc2 proteins in colon tissue of rats was detected by western blotting. Results Jianpi Xiaoke Decoction could effectively reduce fasting blood glucose,down-regulate IL-1β,TNF-αinflammatory factors expression in serum,promote IL-10 content expression,inhibit DAO,D-lactic acid content expression,significantly down-regulate TG,TC,LDL-C expression,but only had a tendency to increase HDL-C expression;meanwhile,Jianpi Xiaoke Decoction could improve pancreatic and ileal injury,increase GLP-1 positive expression,and effectively reduce the relative expression of occludin,ZO-1,Muc2protein in colon of type 2 diabetic rats. Conclusion Jianpi Xiaoke Decoction has a better improving effect on type 2 diabetic rats,which may be related to improving serum inflammation,regulating the expression of lipid content,alleviating intestinal function damage,improving intestinal barrier permeability and protecting intestinal barrier physiological structure.
[1]赵晓娟，杨丽霞，甘德成，等.中医药治疗2型糖尿病糖脂代谢紊乱研究进展[J].亚太传统医药，2023,19(7):234-238.
[2]赵能江，楚可新，王萍，等.杞黄降糖胶囊对2型糖尿病生活质量的改善及相关性分析[J].中华中医药学刊，2023,41(11):1-7.
[3]吴银润.不同尿白蛋白肌酐比值的2型糖尿病患者临床特点和相关危险因素分析[D].大理：大理大学，2023:19-22.
[4]李梓琦.从“脾不散精”论治肥胖2型糖尿病的临床研究[D].长春：长春中医药大学，2023:2-6.
[5]王开欣，张攀，罗涛，等.芍药甘草汤加味联合中药熏洗治疗阴虚血瘀型糖尿病周围神经病变临床研究[J].辽宁中医药大学学报，2023,25(9):212-216.
[6]李剑，高法，曹方，等.从“脾损络伤”探讨2型糖尿病的中医病机[J].中国中医基础医学杂志，2023,29(6):912-915.
[7]罗丹，黄杰，房国伟，等.基于“肠-胰轴”的健脾消渴方对代谢综合征患者胰岛β细胞功能的影响研究[J].中药新药与临床药理，2022,33(8):1118-1123.
[8]路振凯.健脾消渴方治疗T2DM的回顾性研究及分子机制研究[D].济南：山东中医大学，2022:21-23.
[9] GUAN R,MA N,LIU G,et al. Ethanol extract of propolis regulates type 2 diabetes in mice via metabolism and gut microbiota[J]. J Ethnopharmacol,2023,310:116385.
[10] ELSAYED N A,ALEPPO G,ARODA V R,et al. Classification and diagnosis of diabetes:Standards of care in diabetes-2023[J]. Diabetes Care,2023,46(Suppl 1):S19-S40.
[11] ANTZA C,KOSTOPOULOS G,MOSTAFA S,et al. The links between sleep duration,obesity and type 2 diabetes mellitus[J].J Endocrinol,2021,252(2):125-141.
[12] RHODES E T,PROSSER L A,HOERGER T J,et al. Estimated morbidity and mortality in adolescents and young adults diagnosed with type 2 diabetes mellitus[J]. Diabet Med,2012,29(4):453-463.
[13] LAVIE C J,MILANI R V,VENTURA H O. Obesity and cardiovascular disease:Risk factor,paradox,and impact of weight loss[J]. J Am Coll Cardiol,2009,53(21):1925-1932.
[14] ALADHAMI A K,UNGER C A,ENNIS S L,et al. Macrophage tumor necrosis factor-alpha deletion does not protect against obesity-associated metabolic dysfunction[J]. FASEB J,2021,35(7):e21665.
[15] GONCALVES C V,RIBEIRO I S,GALANTINI M P L,et al.Inflammaging and body composition:New insights in diabetic and hypertensive elderly men[J]. Exp Gerontol,2022,170:112005.
[16] ZHANG L,ZHANG Y,LIU J,et al. Association of lipopolysaccharide-Toll-like receptor 4 signaling and microalbuminuria in patients with type 2 diabetes mellitus[J].Diabetes Metab Syndr Obes,2022,15:3143-3152.
[17] SUN J N,HOU B,AI M,et al. The effect of different types of exercise on the intestinal mechanical barrier and related regulatory factors in type 2 diabetic mice[J]. Sheng Li Xue Bao,2022,74(2):237-245.
[18] CAO Y,YAO G,SHENG Y,et al. Jinqi Jiangtang tablet regulates gut microbiota and improve insulin sensitivity in type 2diabetes mice[J]. J Diabetes Res,2019,2019:1872134.
[19] SU M,TANG T,TANG W,et al. Astragalus improves intestinal barrier function and immunity by acting on intestinal microbiota to treat T2DM:A research review[J]. Front Immunol,2023,14:1243834.
[20] HUANG J,GUAN B,LIN L,et al. Improvement of intestinal barrier function,gut microbiota,and metabolic endotoxemia in type 2 diabetes rats by curcumin[J]. Bioengineered,2021,12(2):11947-11958.
[21] REHMAN A U,SIDDIQUI N Z,FAROOQUI N A,et al.Morchella esculenta mushroom polysaccharide attenuates diabetes and modulates intestinal permeability and gut microbiota in a type 2 diabetic mice model[J]. Front Nutr,2022,9:984695.
[22] XU J,LIANG R,ZHANG W,et al. Faecalibacterium prausnitzii-derived microbial anti-inflammatory molecule regulates intestinal integrity in diabetes mellitus mice via modulating tight junction protein expression[J]. J Diabetes,2020,12(3):224-236.
[23] HE L,YANG F Q,TANG P,et al. Regulation of the intestinal flora:A potential mechanism of natural medicines in the treatment of type 2 diabetes mellitus[J]. Biomed Pharmacother,2022,151:113091.
[24] GAI Z,LIAO W,HUANG Y,et al. Effects of Bifidobacterium BL21 and Lacticaseibacillus LRa05 on gut microbiota in type2 diabetes mellitus mice[J]. AMB Express,2023,13(1):97.
基本信息:
DOI：10.13194/j.issn.1673-842x.2024.09.007
中图分类号:R285.5
引用信息:
[1]邓龙飞,顾晶晶,黄珍祯,等.健脾消渴方改善2型糖尿病大鼠药效学研究[J].辽宁中医药大学学报,2024,26(09):32-37.DOI:10.13194/j.issn.1673-842x.2024.09.007.
基金信息:
上海市卫生健康委员会中医药科研项目(2022QN075); 上海市浦东新区科技发展基金民生科研专项项目(PKJ2021-Y26); 上海市浦东新区卫生和计划生育委员会优秀青年医学人才培养项目(PWRq2021-34)