辽宁中医药大学学报

目的实验通过观察基于化痰祛瘀法的自拟中药对冠心病大鼠心肌细胞钙敏感受体的影响,探讨化痰祛瘀法对冠心病大鼠心肌细胞钙敏感受体的影响,以及化痰祛瘀中药治疗冠心病的低、中、高剂量的最优量效比。方法将105只雄性Wistar大鼠,随机选出15只为假手术组,其余90只大鼠予高脂饲料喂养6周后,再配合冠状动脉结扎的方法建立冠心病大鼠模型,将造模成功的大鼠随机分为模型组、西药对照组、中药高剂量组、中药中剂量组、中药低剂量组,用药4周后,观察各组大鼠的血脂总胆固醇(TC)、甘油三酯(TG)、心肌细胞的钙敏感受体蛋白表达及心肌组织形态学的变化。结果与假手术组相比,模型组大鼠血清TG、TC水平明显升高(P<0.05);与模型组相比,中药低、中、高剂量组血清TG、TC水平明显降低(P<0.05);与西药组比较,中药低、中、高组血清TG、TC水平明显降低(P<0.05)。与假手术组相比,模型组的心肌细胞CaSR蛋白表达明显升高(P<0.05);与模型组相比,中药低、中、高剂量组及西药组的心肌细胞CaSR蛋白表达均明显降低(P<0.05);与西药组相比,中药高剂量组能明显降低心肌细胞CaSR蛋白表达,疗效优于西药组(P<0.05),中药中剂量组的疗效等同于西药对照组(P>0.05)。结论化痰祛瘀中药能降低血脂,降低冠心病大鼠的钙敏感受体蛋白表达,对冠心病大鼠具有良好的治疗作用。

Objective To observe the effect of resolving phlegm and removing blood stasis traditional Chinese medicine on calcium sensitive receptor(CaSR)in rats with coronary heart disease,discuss the mechanism of action of resolving phlegm and removing blood stasis method on CaSR in rats with coronary heart disease,and the optimal dose effect ratio of low,medium and high doses of resolving phlegm and removing blood stasis herbs in the treatment of coronary heart disease. Methods From 105 male rats,15 rats were randomly selected as sham operation group,the other 90 rats were fed with high-fat diet. After 6 weeks,coronary artery ligation was used to make them become coronary heart disease rat models. The successful rats were randomly divided into model group,Western medicine control group,Chinese medicine high group,Chinese medicine middle group and Chinese medicine group. After 4 weeks of treatment,TC,TG,calcium sensitive receptor protein expression and myocardial histomorphology were observed. Results Compared with the sham operation group,TG and TC in the model group were significantly increased(P<0.05);compared with the model group,the levels of TG and TC in the low,medium and high groups of traditional Chinese medicine were significantly decreased(P<0.05);compared with the Western medicine group,the levels of TG and TC in the low,medium and high groups of traditional Chinese medicine were significantly decreased(P<0.05). Compared with the sham operation group,the expression of CaSR protein in the model group was significantly increased(P<0.05);compared with the model group,the expression of CaSR protein in the low,medium and high dose groups of traditional Chinese medicine and the Western medicine group were significantly decreased(P<0.05);compared with the Western medicine group,the expression of CaSR protein in the high dose group of Chinese medicine was significantly lower than that in the Western medicine group(P<0.05). The curative effect of the middle dose group was the same as that of the Western medicine control group(P>0.05). Conclusion Resolving phlegm and removing blood stasis traditional Chinese medicine can reduce blood lipid and calcium sensitive receptor protein expression in rats with coronary heart disease,which has a good therapeutic effect on coronary heart disease rats.