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目的:优选延胡索的提取工艺。方法:以延胡索乙素百分含量和转移率为指标,用HPLC测定,考察不同提取工艺、提取次数、乙醇浓度、提取时间、乙醇用量5个因素对延胡索乙素提取含量的影响。结果:延胡索的最佳提取工艺为70%乙醇提取2次,每次3 h,第1次用8倍量醇,第2次用8倍量醇。结论:优选的延胡索提取工艺节省了时间,且简便可行,稳定性较好,提取率为64%。对规范延胡索的提取工艺有一定的借鉴意义。
Abstract：Objective:To optimize the extraction process of Rhizoma Corydalis. Methods:The experiment is measured by HPLC method and tetrahydropalmatine percent content and transfer rate are taken as indexes in this experiment,to analyze the effects of five factors including different extraction process,ethanol concentration,extration times,extration time and amount of ethanol extract to the contents of tetrahydropalmatine. Results:The optimum process of extracting Rhizoma Corydalis is to extract 2 times with 70% ethanol,each time 3 h,the first time use 8 times the amount of alcohol,the second time 8 times the amount of alcohol. Conclusion:The optimal extraction process of Rhizoma Corydalis can save time,and it is simple and feasible with good stability,the extraction rate is sixty-four percent,which have a certain significance for the extraction of Rhizoma Corydalis.
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基本信息:
DOI：10.13194/j.issn.1673-842x.2017.02.015
中图分类号:R284.2
引用信息:
[1]接强,贺吉香.延胡索提取工艺研究[J].辽宁中医药大学学报,2017,19(02):49-52.DOI:10.13194/j.issn.1673-842x.2017.02.015.