辽宁中医药大学学报

目的 探讨抗感方对免疫功能低下小鼠的免疫调节作用及抗H1N1流感病毒作用。方法 采用小鼠网状内皮系统吞噬能力试验（非特异性免疫），小鼠血清溶血素生成试验（体液免疫），小鼠迟发超敏反应试验（细胞免疫），小鼠免疫因子调控试验，观察抗感方对免疫功能低下小鼠的免疫调节作用；采用药物对流感病毒感染BALB/C小鼠死亡的保护实验，观察抗感方抗H1N1流感病毒作用。结果 与模型组比较，各给药组小鼠网状内皮系统吞噬能力有升高的趋势，玉屏风颗粒组（1.95 g/kg）、匹多莫德组（0.21 g/kg）差异有统计学意义（P<0.05或P<0.01）。与模型组比较，各给药组小鼠血清溶血素生成OD值都有升高的趋势，其中抗感方高剂量组（27.30 g生药/kg）、匹多莫德组（0.21 g/kg）升高差异有统计学意义（P<0.05或P<0.01）。与模型组比较，各给药组小鼠迟发超敏反应有下降的趋势，其中抗感方低剂量组（6.82 g生药/kg）、高剂量组（27.30 g生药/kg）、匹多莫德组（0.21 g/kg）降低明显（P<0.05或P<0.01）。与模型对照组比较，抗感方高剂量组（27.30 g生药/kg）、玉屏风颗粒组（1.95 g/kg）、匹多莫德组（0.21 g/kg）能够促进IL-4分泌（P<0.05或P<0.01），抗感方高剂量组（27.30 g生药/kg）、匹多莫德组（0.21 g/kg）能够促进IFN-γ的分泌（P<0.01、P<0.05），抗感方高剂量组（27.30 g生药/kg）能够抑制TNF-α的分泌（P<0.05）。与模型组比较，抗感方中剂量组（13.65 g生药/kg）、奥司他韦胶囊组（19.50 mg/kg）平均生存日数延长（P<0.05或P<0.01）。结论 抗感方具有提高机体免疫力及抗H1N1流感病毒作用，其机制可能与促进IL-4、IFN-γ分泌，抑制TNF-α的生成有关。

Objective To investigate the immunoregulatory effect of Kanggan Decoction（抗感方） on immunocompromised mice and its anti-H1N1 influenza virus effect. Methods To observe the immunomodulatory effect of Kanggan Decoction on immunocompromised mice via the following tests as the reticuloendothelial system phagocytosis test(non-specific immunity),serum hemolysin production test(humoral immunity),delayed hypersensitivity test(cellular immunity)and immune factor regulation test. The protective effect of anti-H1N1 influenza virus was observed by the protective test of drugs on the death of BALB/C mice infected with influenza virus. Results Compared with the model group,the phagocytic ability of the reticuloendothelial system of the mice in each administration group tended to increase,and the difference between the Yupingfeng Granule （玉屏风颗粒）group(1.95 g/kg)and the pidotimod group(0.21 g/kg) was statistically significant(P<0.05 or P<0.01). Compared with the model group,the OD value of serum hemolysin production in each administration group showed an increasing trend,among which the high-dose group of Kanggan Decoction(27.30 g crude drug/kg)and the pidotimod group(0.21 g/kg)increased significantly(P<0.05 or P<0.01). Compared with the model group,the delayed hypersensitivity of mice in each administration group showed a downward trend,among which the low-dose group of Kanggan Decoction(6.82 g crude drug/kg),high dose group(27.30 g crude drug/kg),pidotimod group(0.21 g/kg)decreased significantly(P<0.05 or P<0.01). Compared with the model control group,the high dose group(27.30 g crude drug/kg),Yupingfeng Granule group(1.95 g/kg)and pidotimod group(0.21 g/kg)could promote the secretion of IL-4(P<0.05 or P<0.01),the high dose group(27.30 g crude drug/kg)and pidotimod group(0.21 g/kg)could promote the secretion of IFN-γ(P<0.01,P<0.05),and the high dose group(27.30 g crude drug/kg)could inhibit the secretion of TNF-α(P<0.05). Compared with the model group,the average survival days of the medium dose group(13.65 g crude drug/kg)and the oseltamivir capsule group(19.50 mg/kg)were prolonged(P<0.05 or P<0.01). Conclusion Kanggan Decoction has the effect of improving immunity and anti-H1N1 influenza virus,and its mechanism may be related to promoting the secretion of IL-4 and IFN-γ and inhibiting the production of TNF-α.